4uoq: Difference between revisions
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''' | ==Nucleophile mutant (E324A) of beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04== | ||
<StructureSection load='4uoq' size='340' side='right' caption='[[4uoq]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4uoq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UOQ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uoz|4uoz]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uoq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uoq RCSB], [http://www.ebi.ac.uk/pdbsum/4uoq PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Bifidobacterium genus harbours several health promoting members of the gut microbiota. Bifidobacteria display metabolic specialization by preferentially utilizing dietary or host derived beta-galactosides. This study investigates the biochemistry and structure of a glycoside hydrolase family 42 (GH42) beta-galactosidase from the probiotic Bifidobacterium animalis subsp. lactis Bl-04 (BlGal42A). BlGal42A displays a preference for undecorated beta1-6 and beta1-3 linked galactosides and populates a phylogenetic cluster with close bifidobacterial homologues implicated in the utilization of N-acetyl substituted beta1-3 galactosides from human milk and mucin. A long loop containing an invariant tryptophan in GH42, proposed to bind substrate at subsite +1, is identified here as specificity signature within this clade of bifidobacterial enzymes. Galactose binding at the subsite -1 of the active site induced conformational changes resulting in an extra polar interaction and the ordering of a flexible loop that narrows the active site. The amino-acid sequence of this loop provides an additional specificity signature within this GH42 clade. The phylogenetic relatedness of enzymes targeting beta1-6 and beta1-3 galactosides likely reflects structural differences between these substrates and beta1-4 galactosides, containing an axial galactosidic bond. These data advance our molecular understanding of the evolution of sub-specificities that support metabolic specialization in the gut niche. | |||
A beta1-6/beta1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of beta-galactoside catabolism within Bifidobacterium.,Viborg AH, Fredslund F, Katayama T, Nielsen SK, Svensson B, Kitaoka M, Leggio LL, Abou Hachem M Mol Microbiol. 2014 Oct 7. doi: 10.1111/mmi.12815. PMID:25287704<ref>PMID:25287704</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Beta-galactosidase]] | |||
[[Category: Fredslund, F.]] | |||
[[Category: Hachem, M Abou.]] | |||
[[Category: Katayama, T.]] | |||
[[Category: Kitaoka, M.]] | |||
[[Category: Leggio, L Lo.]] | |||
[[Category: Nielsen, S K.]] | |||
[[Category: Svensson, B.]] | |||
[[Category: Viborg, A H.]] | |||
[[Category: Gh42]] | |||
[[Category: Hydrolase]] | |||
Revision as of 12:29, 22 October 2014
Nucleophile mutant (E324A) of beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04
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