1is8: Difference between revisions
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|PDB= 1is8 |SIZE=350|CAPTION= <scene name='initialview01'>1is8</scene>, resolution 2.70Å | |PDB= 1is8 |SIZE=350|CAPTION= <scene name='initialview01'>1is8</scene>, resolution 2.70Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1is7|1IS7]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1is8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1is8 OCA], [http://www.ebi.ac.uk/pdbsum/1is8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1is8 RCSB]</span> | |||
}} | }} | ||
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[[Category: Maita, N.]] | [[Category: Maita, N.]] | ||
[[Category: Okada, K.]] | [[Category: Okada, K.]] | ||
[[Category: enzyme-regulatory protein complex]] | [[Category: enzyme-regulatory protein complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:55 2008'' | ||
Revision as of 18:23, 30 March 2008
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| 1is8, resolution 2.70Å | |||||||||||||
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| Ligands: | K, PHE, ZN | ||||||||||||
| Activity: | GTP cyclohydrolase I, with EC number 3.5.4.16 | ||||||||||||
| Related: | 1IS7
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of rat GTPCHI/GFRP stimulatory complex plus Zn
Overview
In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state.
About this Structure
1IS8 is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP., Maita N, Okada K, Hatakeyama K, Hakoshima T, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1212-7. Epub 2002 Jan 29. PMID:11818540
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