1xhp

Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae

spinqualitylabelsall models 1xhp, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1xhp is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1sy4, 1r2p, 1kxk Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site. U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction.,Sashital DG, Cornilescu G, McManus CJ, Brow DA, Butcher SE Nat Struct Mol Biol. 2004 Dec;11(12):1237-42. Epub 2004 Nov 14. PMID:15543154 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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