1wac

BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE

spinqualitylabelsall models 1wac, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1wac is a 3 chain structure with sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1hhs, 1hht, 1hi0, 1hi1, 1hi8, 1uvi, 1uvj, 1uvk, 1uvl, 1uvm, 1uvn Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods. Back-priming mode of phi6 RNA-dependent RNA polymerase.,Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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