2erl

PHEROMONE ER-1 FROM

spinqualitylabelsall models 2erl, resolution 1.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2erl is a 1 chain structure with sequence from Euplotes raikovi. This structure supersedes the now removed PDB entry 1erl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EOH Resources: FirstGlance, OCA, RCSB, PDBsum Function [MER1_EUPRA] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. The membrane-bound form promotes inter-cellular communication and adhesion for mating pair formation and may act as binding site for the secreted form. Publication Abstract from PubMed Four different phasing methods have been applied to the determination of the crystal structure of the 40 amino-acid mating pheromone of the unicellular ciliated protozoan Euplotes raikovi. The difficulties, failures and successes in attempts to solve the structure by: (1) molecular replacement, (2) direct phasing using the 'Shake and Bake' algorithm, (3) isomorphous replacement, and (4) multiple-wavelength anomalous dispersion are described. The structure was first solved by molecular replacement, and then was the first successful structure determination by 'Shake and Bake' without the direct involvement of its authors. A description of the current status of the high-resolution refinement of the structure is also given. The model is refined against 1 A resolution data to an R factor of 12.9%, and includes H atoms and discretely disordered side chains. A challenging case for protein crystal structure determination: the mating pheromone Er-1 from Euplotes raikovi.,Anderson DH, Weiss MS, Eisenberg D Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):469-80. PMID:15299668 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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