Structural highlights
2kf7 is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| NonStd Res: | BGM |
| Related: | 143d, 1k8p, 2gku, 2aqy, 2jsk, 2jsl, 2jsm, 2jsq, 2kf8 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
Previously, it has been reported that human telomeric DNA sequences could adopt in different experimental conditions four different intramolecular G-quadruplexes each involving three G-tetrad layers, namely, Na(+) solution antiparallel-stranded basket form, K(+) crystal parallel-stranded propeller form, K(+) solution (3 + 1) Form 1, and K(+) solution (3 + 1) Form 2. Here we present a new intramolecular G-quadruplex adopted by a four-repeat human telomeric sequence in K(+) solution (Form 3). This structure is a basket-type G-quadruplex with only two G-tetrad layers: loops are successively edgewise, diagonal, and edgewise; glycosidic conformations of guanines are syn*syn*anti*anti around each tetrad. Each strand of the core has both a parallel and an antiparallel adjacent strands; there are one narrow, one wide, and two medium grooves. Despite the presence of only two G-tetrads in the core, this structure is more stable than the three-G-tetrad intramolecular G-quadruplexes previously observed for human telomeric sequences in K(+) solution. Detailed structural elucidation of Form 3 revealed extensive base pairing and stacking in the loops capping both ends of the G-tetrad core, which might explain the high stability of the structure. This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA.
Structure of the Human Telomere in K(+) Solution: A Stable Basket-Type G-Quadruplex with Only Two G-Tetrad Layers.,Lim KW, Amrane S, Bouaziz S, Xu W, Mu Y, Patel DJ, Luu KN, Phan AT J Am Chem Soc. 2009 Mar 9. PMID:19271707[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lim KW, Amrane S, Bouaziz S, Xu W, Mu Y, Patel DJ, Luu KN, Phan AT. Structure of the Human Telomere in K(+) Solution: A Stable Basket-Type G-Quadruplex with Only Two G-Tetrad Layers. J Am Chem Soc. 2009 Mar 9. PMID:19271707 doi:10.1021/ja807503g
