2o1i

RH(BPY)2CHRYSI complexed to mismatched DNA

spinqualitylabelsall models 2o1i, resolution 1.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2o1i is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: R1C Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed We report the 1.1-A resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in the oligonucleotide 5'-(dCGGAAATTCCCG)2-3'. At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized. This unique binding mode contrasts with major groove intercalation, observed at a matched site, where doubling of the base pair rise accommodates stacking of the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches. Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator.,Pierre VC, Kaiser JT, Barton JK Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):429-34. Epub 2006 Dec 28. PMID:17194756 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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