4rns: Difference between revisions

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'''Unreleased structure'''
==PcpR inducer binding domain (apo-form)==
<StructureSection load='4rns' size='340' side='right' caption='[[4rns]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rns]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNS FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rns RCSB], [http://www.ebi.ac.uk/pdbsum/4rns PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PcpR is a LysR-type transcription factor from Sphingobium chlorophenolicum L-1 that is responsible for the activation of several genes involved in polychlorophenol degradation. PcpR responds to several polychlorophenols in vivo. Here, we report the crystal structures of the inducer-binding domain of PcpR in the apo-form and binary complexes with pentachlorophenol (PCP) and 2,4,6-trichlorophenol (2,4,6-TCP). Both X-ray crystal structures and isothermal titration calorimetry data indicated the association of two PCP molecules per PcpR, but only one 2,4,6-TCP molecule. The hydrophobic nature and hydrogen bonds of one binding cavity allowed the tight association of both PCP (Kd = 110 nM) and 2,4,6-TCP (Kd = 22.8 nM). However, the other cavity was unique to PCP with much weaker affinity (Kd = 70 muM) and thus its significance was not clear. Neither phenol nor benzoic acid displayed any significant affinity to PcpR, indicating a role of chlorine substitution in ligand specificity. When PcpR is compared with TcpR, a LysR-type regulator controlling the expression of 2,4,6-trichlorophenol degradation in Cupriavidus necator JMP134, most of the residues constituting the two inducer-binding cavities of PcpR are different, except for their general hydrophobic nature. The finding concurs that PcpR uses various polychlorophenols as long as it includes 2,4,6-trichlorophenol, as inducers; whereas TcpR is only responsive to 2,4,6-trichlorophenol.


The entry 4rns is ON HOLD  until Paper Publication
Structures of the Inducer-Binding Domain of Pentachlorophenol-Degrading Gene Regulator PcpR from Sphingobium chlorophenolicum.,Hayes RP, Moural TW, Lewis KM, Onofrei D, Xun L, Kang C Int J Mol Sci. 2014 Nov 12;15(11):20736-52. doi: 10.3390/ijms151120736. PMID:25397598<ref>PMID:25397598</ref>


Authors: Hayes, R.P., Moural, T.W., Lewis, K.M., Onofrei, D., Xun, L., Kang, C.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: PcpR inducer binding domain (apo-form)
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hayes, R P]]
[[Category: Kang, C]]
[[Category: Lewis, K M]]
[[Category: Moural, T W]]
[[Category: Onofrei, D]]
[[Category: Xun, L]]
[[Category: Lysr family transcriptional regulator]]
[[Category: Transcription]]
Retrieved from "https://proteopedia.org/4rns"