3crf

Electron Microscopy model of the Saf Pilus- Type B

spinqualitylabelsall models 3crf, resolution 17.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3crf is a 3 chain structure with sequence from Salmonella enterica subsp. enterica serovar typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2co4, 3cre Gene: safA (Salmonella enterica subsp. enterica serovar Typhimurium) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Bacterial pili are important virulence factors involved in host cell attachment and/or biofilm formation, key steps in establishing and maintaining successful infection. Here we studied Salmonella atypical fimbriae (or Saf pili), formed by the conserved chaperone/usher pathway. In contrast to the well-established quaternary structure of typical/FGS-chaperone assembled, rod-shaped, chaperone/usher pili, little is known about the supramolecular organisation in atypical/FGL-chaperone assembled fimbriae. In our study, we have used negative stain electron microscopy and single-particle image analysis to determine the three-dimensional structure of the Salmonella typhimurium Saf pilus. Our results show atypical/FGL-chaperone assembled fimbriae are composed of highly flexible linear multi-subunit fibres that are formed by globular subunits connected to each other by short links giving a "beads on a string"-like appearance. Quantitative fitting of the atomic structure of the SafA pilus subunit into the electron density maps, in combination with linker modelling and energy minimisation, has enabled analysis of subunit arrangement and intersubunit interactions in the Saf pilus. Short intersubunit linker regions provide the molecular basis for flexibility of the Saf pilus by acting as molecular hinges allowing a large range of movement between consecutive subunits in the fibre. Structural analysis of the Saf pilus by electron microscopy and image processing.,Salih O, Remaut H, Waksman G, Orlova EV J Mol Biol. 2008 May 23;379(1):174-87. Epub 2008 Apr 3. PMID:18448124 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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