2v0r: Difference between revisions

The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease, encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic, integration of new L1 and Alu elements that presently account for, approximately 28% of the human genome. L1-EN bears considerable, technological interest, because its target selectivity may ultimately be, engineered to allow the site-specific integration of DNA into defined, genomic locations. Based on the crystal structure, we generated L1-EN, mutants to analyze and manipulate DNA target site recognition. Crystal, structures and their dynamic and functional analysis show entire loop, grafts to be feasible, resulting in altered specificity, while individual, point mutations do not change the nicking pattern of L1-EN. Structural, parameters of the DNA target seem more important for recognition than the, nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro, are less well defined than the respective integration site consensus in, vivo. This suggests that additional factors other than the DNA nicking, specificity of L1-EN contribute to the targeted integration of non-LTR, retrotransposons.

2V0R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V0R OCA].  

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