4wsi: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Crystal Structure of PALS1/Crb complex==

== Structural highlights ==

<table><tr><td colspan='2'>[[4wsi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WSI FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wsi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wsi RCSB], [http://www.ebi.ac.uk/pdbsum/4wsi PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The Crumbs (Crb) complex, formed by Crb, PALS1, and PATJ, is evolutionarily conserved in metazoans and acts as a master cell-growth and -polarity regulator at the apical membranes in polarized epithelia. Crb intracellular functions, including its direct binding to PALS1, are mediated by Crb's highly conserved 37-residue cytoplasmic tail. However, the mechanistic basis governing the highly specific Crb-PALS1 complex formation is unclear, as reported interaction between the Crb tail (Crb-CT) and PALS1 PSD-95/DLG/ZO-1 (PDZ) domain is weak and promiscuous. Here we have discovered that the PDZ-Src homolgy 3 (SH3)-Guanylate kinase (GK) tandem of PALS1 binds to Crb-CT with a dissociation constant of 70 nM, which is approximately 100-fold stronger than the PALS1 PDZ-Crb-CT interaction. The crystal structure of the PALS1 PDZ-SH3-GK-Crb-CT complex reveals that PDZ-SH3-GK forms a structural supramodule with all three domains contributing to the tight binding to Crb. Mutations disrupting the tertiary interactions of the PDZ-SH3-GK supramodule weaken the PALS1-Crb interaction and compromise PALS1-mediated polarity establishment in Madin-Darby canine kidney (MDCK) cysts. We further show that specific target binding of other members of membrane-associated guanylate kinases (MAGUKs) (e.g., CASK binding to neurexin) also requires the presence of their PDZ-SH3-GK tandems.

~~The entry 4wsi is ON HOLD until Paper Publication~~

Structure of Crumbs tail in complex with the PALS1 PDZ-SH3-GK tandem reveals a highly specific assembly mechanism for the apical Crumbs complex.,Li Y, Wei Z, Yan Y, Wan Q, Du Q, Zhang M Proc Natl Acad Sci U S A. 2014 Nov 10. pii: 201416515. PMID:25385611<ref>PMID:25385611</ref>

~~Authors: Wei~~, Z., Li, Y., Zhang, M.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description~~: ~~Crystal Structure of PALS1/Crb complex~~

== References ==

__TOC__

</StructureSection>

[[Category: Li, Y]]

[[Category: Wei, Z]]

[[Category: Zhang, M]]

[[Category: Peptide binding protein]]

[[Category: Supramodule]]

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-'''Unreleased structure'''
+==Crystal Structure of PALS1/Crb complex==
+<StructureSection load='4wsi' size='340' side='right' caption='[[4wsi]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4wsi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WSI FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wsi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wsi RCSB], [http://www.ebi.ac.uk/pdbsum/4wsi PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Crumbs (Crb) complex, formed by Crb, PALS1, and PATJ, is evolutionarily conserved in metazoans and acts as a master cell-growth and -polarity regulator at the apical membranes in polarized epithelia. Crb intracellular functions, including its direct binding to PALS1, are mediated by Crb's highly conserved 37-residue cytoplasmic tail. However, the mechanistic basis governing the highly specific Crb-PALS1 complex formation is unclear, as reported interaction between the Crb tail (Crb-CT) and PALS1 PSD-95/DLG/ZO-1 (PDZ) domain is weak and promiscuous. Here we have discovered that the PDZ-Src homolgy 3 (SH3)-Guanylate kinase (GK) tandem of PALS1 binds to Crb-CT with a dissociation constant of 70 nM, which is approximately 100-fold stronger than the PALS1 PDZ-Crb-CT interaction. The crystal structure of the PALS1 PDZ-SH3-GK-Crb-CT complex reveals that PDZ-SH3-GK forms a structural supramodule with all three domains contributing to the tight binding to Crb. Mutations disrupting the tertiary interactions of the PDZ-SH3-GK supramodule weaken the PALS1-Crb interaction and compromise PALS1-mediated polarity establishment in Madin-Darby canine kidney (MDCK) cysts. We further show that specific target binding of other members of membrane-associated guanylate kinases (MAGUKs) (e.g., CASK binding to neurexin) also requires the presence of their PDZ-SH3-GK tandems.
-The entry 4wsi is ON HOLD  until Paper Publication
+Structure of Crumbs tail in complex with the PALS1 PDZ-SH3-GK tandem reveals a highly specific assembly mechanism for the apical Crumbs complex.,Li Y, Wei Z, Yan Y, Wan Q, Du Q, Zhang M Proc Natl Acad Sci U S A. 2014 Nov 10. pii: 201416515. PMID:25385611<ref>PMID:25385611</ref>
-Authors: Wei, Z., Li, Y., Zhang, M.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal Structure of PALS1/Crb complex
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Li, Y]]
+[[Category: Wei, Z]]
+[[Category: Zhang, M]]
+[[Category: Peptide binding protein]]
+[[Category: Supramodule]]