DNA ligase: Difference between revisions

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'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mammalian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining. ~~LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif.~~ See more details in [[ATP-Dependent DNA Ligase (Bacteriophage T7)]].

'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated, in a species-dependent manner, by hydrolysis of ATP or NAD+.<br />

* Mammalian '''LigD I''' ligates the nascent DNA of the lagging strand.<br />

* '''LigD III''' complexes with XRCC1 in the process of nucleotide excision repair.<br />

* '''LigD IV''' complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.<br />

See more details in [[ATP-Dependent DNA Ligase (Bacteriophage T7)]].

==Disease==

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A specific point mutation (46BR in the mouse Lig1 gene) in DNA ligase I has also been linked as a cause for genome instability and cancer in humans. One possible reason for this is the accumulation of DNA fragments that are no longer ligated by the mutant DNA ligase I enzyme.

== Structural highlights ==

LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif.

</StructureSection>

DNA ligase: Difference between revisions

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 <StructureSection load='2hix' size='400' side='right' scene='DNA_ligase/Cv/1' caption='ATP-dependent DNA ligase complexed with ATP [[2hix]]'>
-'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA.  LigD is activated , in a species-dependent manner, by hydrolysis of  ATP or NAD+.  Mammalian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.  LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif.  See more details in [[ATP-Dependent DNA Ligase (Bacteriophage T7)]].
+'''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA.  LigD is activated, in a species-dependent manner, by hydrolysis of  ATP or NAD+.<br />
+* Mammalian '''LigD I''' ligates the nascent DNA of the lagging strand.<br />
+* '''LigD III''' complexes with XRCC1 in the process of nucleotide excision repair.<br />
+* '''LigD IV''' complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.<br />
+See more details in [[ATP-Dependent DNA Ligase (Bacteriophage T7)]].
 ==Disease==
@@ Line 8: / Line 12: @@
 A specific point mutation (46BR in the mouse Lig1 gene) in DNA ligase I has also been linked as a cause for genome instability and cancer in humans. One possible reason for this is the accumulation of DNA fragments that are no longer ligated by the mutant DNA ligase I enzyme.
+== Structural highlights ==
+LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif.
 </StructureSection>