3j8x: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "3j8x" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
''' | ==High-resolution structure of no-nucleotide kinesin on microtubules== | ||
<StructureSection load='3j8x' size='340' side='right' caption='[[3j8x]], [[Resolution|resolution]] 5.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3j8x]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J8X FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j8y|3j8y]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j8x RCSB], [http://www.ebi.ac.uk/pdbsum/3j8x PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Microtubule-based transport by the kinesin motors, powered by ATP hydrolysis, is essential for a wide range of vital processes in eukaryotes. We obtained insight into this process by developing atomic models for no-nucleotide and ATP states of the monomeric kinesin motor domain on microtubules from cryo-EM reconstructions at 5-6A resolution. By comparing these models with existing X-ray structures of ADP-bound kinesin, we infer a mechanistic scheme in which microtubule attachment, mediated by a universally conserved 'linchpin' residue in kinesin (N255), triggers a clamshell opening of the nucleotide cleft and accompanying release of ADP. Binding of ATP re-closes the cleft in a manner that tightly couples to translocation of cargo, via kinesin's 'neck linker' element. These structural transitions are reminiscent of the analogous nucleotide-exchange steps in the myosin and F1-ATPase motors, and inform how the two heads of a kinesin dimer 'gate' each other to promote coordinated stepping along microtubules. | |||
High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force generation.,Shang Z, Zhou K, Xu C, Csencsits R, Cochran JC, Sindelar CV Elife. 2014 Nov 21;3. doi: 10.7554/eLife.04686. PMID:25415053<ref>PMID:25415053</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Sus scrofa]] | |||
[[Category: Cochran, J C]] | |||
[[Category: Csencsits, R]] | |||
[[Category: Shang, Z]] | |||
[[Category: Sindelar, C V]] | |||
[[Category: Xu, C]] | |||
[[Category: Zhou, K]] | |||
[[Category: Cytoskeletal motor]] | |||
[[Category: Kinesin]] | |||
[[Category: Microtubule]] | |||
[[Category: Molecular motor]] | |||
[[Category: Motor protein-structural protein complex]] | |||
[[Category: Myosin]] | |||
Revision as of 16:13, 10 December 2014
High-resolution structure of no-nucleotide kinesin on microtubules
| ||||||||||||
