3ftj

Crystal structure of the periplasmic region of MacB from Actinobacillus actinomycetemcomitans

spinqualitylabelsall models 3ftj, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ftj is a 1 chain structure with sequence from Aggregatibacter actinomycetemcomitans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: macB, MACB_ACTAC (Aggregatibacter actinomycetemcomitans) Resources: FirstGlance, OCA, RCSB, PDBsum Function [MACB_ACTAC] Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.[HAMAP-Rule:MF_01720] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which is responsible both for the efflux of macrolide antibiotics and for the secretion of heat-stable enterotoxin II. In Escherichia coli, MacB requires the membrane fusion protein MacA and the multifunctional outer membrane channel TolC to pump substrates to the external medium. Sequence analysis of MacB suggested that MacB has a relatively large periplasmic region. To gain insight into how MacB assembles with MacA and TolC, we determined the crystal structure of the periplasmic region of Actinobacillus actinomycetemcomitans MacB. Fold matching program reveals that parts of the MacB periplasmic region have structural motifs in common with the RND-type transporter AcrB. Since it behaved as a monomer in solution, our finding is consistent with the dimeric nature of full-length MacB, providing an insight into the assembly in the tripartite efflux pump. Crystal Structure of the Periplasmic Region of MacB, a Noncanonic ABC Transporter (,).,Xu Y, Sim SH, Nam KH, Jin XL, Kim HM, Hwang KY, Lee K, Ha NC Biochemistry. 2009 May 21. PMID:19432486 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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