5eau: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have, been a source of these natural products by providing a homologous set of, terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with, two farnesyl diphosphate analogs were analyzed. These structures reveal an, unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the, stereochemical selectivity displayed by other cyclases in the biosynthesis, of pharmacologically important cyclic terpenes. As such, these structures, provide templates for the engineering of novel terpene . | Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have, been a source of these natural products by providing a homologous set of, terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with, two farnesyl diphosphate analogs were analyzed. These structures reveal an, unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the, stereochemical selectivity displayed by other cyclases in the biosynthesis, of pharmacologically important cyclic terpenes. As such, these structures, provide templates for the engineering of novel terpene cyclases. | ||
==About this Structure== | ==About this Structure== | ||
5EAU is a | 5EAU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with MG and FFF as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: MGA and MGB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5EAU OCA]. | ||
==Reference== | ==Reference== | ||
| Line 25: | Line 25: | ||
[[Category: natural products biosynthesis]] | [[Category: natural products biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:05:10 2007'' | ||
