5pgm: Difference between revisions
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==Overview== | ==Overview== | ||
The structure of a new crystal form of Saccharomyces cerevisiae, phosphoglycerate mutase has been solved and refined to 2.12 A with working, and free R-factors of 19.7 and 22.9 %, respectively. Higher-resolution, data and greater non-crystallographic symmetry have produced a more, accurate protein structure than previously. Prominent among the, differences from the previous structure is the presence of two sulphate, ions within each active site cleft. The separation of the sulphates, suggests that they may occupy the same sites as phospho groups of the, bisphosphate ligands of the enzyme. Plausible binding modes for, 2,3-bisphosphoglycerate and 1, 3-bisphosphoglycerate are thereby, suggested. These results support previous conclusions from mutant studies, highlight interesting new targets . | The structure of a new crystal form of Saccharomyces cerevisiae, phosphoglycerate mutase has been solved and refined to 2.12 A with working, and free R-factors of 19.7 and 22.9 %, respectively. Higher-resolution, data and greater non-crystallographic symmetry have produced a more, accurate protein structure than previously. Prominent among the, differences from the previous structure is the presence of two sulphate, ions within each active site cleft. The separation of the sulphates, suggests that they may occupy the same sites as phospho groups of the, bisphosphate ligands of the enzyme. Plausible binding modes for, 2,3-bisphosphoglycerate and 1, 3-bisphosphoglycerate are thereby, suggested. These results support previous conclusions from mutant studies, highlight interesting new targets for mutagenesis and suggest a possible, mechanism of enzyme phosphorylation. | ||
==About this Structure== | ==About this Structure== | ||
5PGM is a | 5PGM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 and ALA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoglycerate_mutase Phosphoglycerate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.1 5.4.2.1] Structure known Active Sites: CIA, CIB, CIC, CID, CIE, CIF, CIG and CIH. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5PGM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase (phosphoryl)]] | [[Category: transferase (phosphoryl)]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:51:01 2007'' | ||
