2v1u: Difference between revisions
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==Overview== | ==Overview== | ||
DNA replication in archaea and in eukaryotes share many similarities. We, report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in, multiple copies at replication origins. DNA binding is mediated, principally by a C-terminal winged helix domain that inserts deeply into, the major and minor grooves, widening them both. However, additional DNA, contacts are made with the N-terminal AAA+ domain, which inserts into the, minor groove at a characteristic G-rich sequence, inducing a 35 degrees, bend in the duplex and providing directionality to the binding site. Both, contact regions also induce substantial unwinding of the DNA. The, structure provides insight into the initial step in assembly of a, | DNA replication in archaea and in eukaryotes share many similarities. We, report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in, multiple copies at replication origins. DNA binding is mediated, principally by a C-terminal winged helix domain that inserts deeply into, the major and minor grooves, widening them both. However, additional DNA, contacts are made with the N-terminal AAA+ domain, which inserts into the, minor groove at a characteristic G-rich sequence, inducing a 35 degrees, bend in the duplex and providing directionality to the binding site. Both, contact regions also induce substantial unwinding of the DNA. The, structure provides insight into the initial step in assembly of a, replication origin and recruitment of minichromosome maintenance (MCM), helicase to that origin. | ||
==About this Structure== | ==About this Structure== | ||
2V1U is a | 2V1U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V1U OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: replication]] | [[Category: replication]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:52:14 2007'' | ||
