1nco: Difference between revisions
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|PDB= 1nco |SIZE=350|CAPTION= <scene name='initialview01'>1nco</scene>, resolution 1.8Å | |PDB= 1nco |SIZE=350|CAPTION= <scene name='initialview01'>1nco</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene> | |LIGAND= <scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nco OCA], [http://www.ebi.ac.uk/pdbsum/1nco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nco RCSB]</span> | |||
}} | }} | ||
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[[Category: Myers, A G.]] | [[Category: Myers, A G.]] | ||
[[Category: Rees, D C.]] | [[Category: Rees, D C.]] | ||
[[Category: antibacterial and antitumor protein]] | [[Category: antibacterial and antitumor protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:28:32 2008'' | ||
Revision as of 19:28, 30 March 2008
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| 1nco, resolution 1.8Å | |||||||||||||
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| Ligands: | CHR, MRD | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN
Overview
Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation.
About this Structure
1NCO is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:8235619
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