1nxu: Difference between revisions
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|PDB= 1nxu |SIZE=350|CAPTION= <scene name='initialview01'>1nxu</scene>, resolution 1.80Å | |PDB= 1nxu |SIZE=350|CAPTION= <scene name='initialview01'>1nxu</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= YIAK OR B3575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= YIAK OR B3575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02615 Ldh_2]</span> | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nxu OCA], [http://www.ebi.ac.uk/pdbsum/1nxu PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1nxu RCSB]</span> | |||
}} | }} | ||
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[[Category: Tong, L.]] | [[Category: Tong, L.]] | ||
[[Category: Xiao, R.]] | [[Category: Xiao, R.]] | ||
[[Category: hypothetical protein]] | [[Category: hypothetical protein]] | ||
[[Category: nesg]] | [[Category: nesg]] | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:59:26 2008'' | ||
Revision as of 03:59, 26 March 2008
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| 1nxu, resolution 1.80Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MSE, SO4 | ||||||||||||
| Gene: | YIAK OR B3575 (Escherichia coli) | ||||||||||||
| Domains: | Ldh_2 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF E. COLI HYPOTHETICAL OXIDOREDUCTASE YIAK NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER82.
Overview
Escherichia coli YiaK catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH. It belongs to a large family of oxidoreductases that is conserved in archaea, bacteria, and eukaryotes but shows no sequence homology to other proteins. We report here the crystal structures at up to 2.0-A resolution of YiaK alone and in complex with NAD-tartrate. YiaK has a new polypeptide backbone fold and a novel mode of recognizing the NAD cofactor. In addition, NAD is bound in an unusual conformation, at the interface of a dimer of the enzyme. The crystallographic analysis unexpectedly revealed the binding of tartrate in the active site. Enzyme kinetics studies confirm that tartrate and the related D-malate are inhibitors of YiaK. In contrast to most other enzymes where substrate binding produces a more closed conformation, the binding of NAD-tartrate to YiaK produces a more open active site. The free enzyme conformation is incompatible with NAD binding. His(44) is likely the catalytic residue of the enzyme.
About this Structure
1NXU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK)., Forouhar F, Lee I, Benach J, Kulkarni K, Xiao R, Acton TB, Montelione GT, Tong L, J Biol Chem. 2004 Mar 26;279(13):13148-55. Epub 2004 Jan 12. PMID:14718529
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Escherichia coli
- Single protein
- Acton, T B.
- Benach, J.
- Forouhar, F.
- Kulkarni, K.
- Lee, I.
- Montelione, G T.
- NESG, Northeast Structural Genomics Consortium.
- Rost, B.
- Shastry, R.
- Tong, L.
- Xiao, R.
- Hypothetical protein
- Nesg
- Northeast structural genomics consortium
- Oxidoreductase
- Protein structure initiative
- Psi
- Structural genomic
