1oar: Difference between revisions
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|PDB= 1oar |SIZE=350|CAPTION= <scene name='initialview01'>1oar</scene>, resolution 2.23Å | |PDB= 1oar |SIZE=350|CAPTION= <scene name='initialview01'>1oar</scene>, resolution 2.23Å | ||
|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+N'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+N'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [http://www.ebi.ac.uk/pdbsum/1oar PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB]</span> | |||
}} | }} | ||
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[[Category: Roversi, P.]] | [[Category: Roversi, P.]] | ||
[[Category: Tawfik, D.]] | [[Category: Tawfik, D.]] | ||
[[Category: allergy]] | [[Category: allergy]] | ||
[[Category: antibody]] | [[Category: antibody]] | ||
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[[Category: multispecificity]] | [[Category: multispecificity]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:42:37 2008'' | ||
Revision as of 19:42, 30 March 2008
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| 1oar, resolution 2.23Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | AC1 | ||||||||||||
| Ligands: | AZN, CAC, CL, DMS, EDO, NA | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED
Overview
A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.
About this Structure
1OAR is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.
Reference
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298
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