3vyc

Crystal structure of unliganded Saccharomyces cerevisiae CRM1 (Xpo1p)

spinqualitylabelsall models 3vyc, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3vyc is a 1 chain structure with sequence from Saccharomyces cerevisiae s288c. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CRM1 (Saccharomyces cerevisiae S288c) Resources: FirstGlance, OCA, RCSB, PDBsum Function [XPO1_YEAST] Receptor for the leucine-rich nuclear export signal (NES). Publication Abstract from PubMed CRM1 mediates nuclear export of numerous proteins and ribonucleoproteins containing a leucine-rich nuclear export signal (NES). Binding of RanGTP to CRM1 in the nucleus stabilizes cargo association with CRM1, and vice versa, but the mechanism underlying the positive cooperativity in RanGTP and NES binding to CRM1 remains incompletely understood. Herein we report a 2.1-A-resolution crystal structure of unliganded Saccharomyces cerevisiae CRM1 (Xpo1p) that demonstrates that an internal loop of CRM1 (referred to as HEAT9 loop) is primarily responsible for maintaining the NES-binding cleft in a closed conformation, rendering CRM1 incapable of NES binding in the absence of RanGTP. The structure also shows that the C-terminal tail of CRM1 stabilizes the autoinhibitory conformation of the HEAT9 loop and thereby reinforces autoinhibition. Comparison with the structures of CRM1-NES-RanGTP complexes reveals how binding of RanGTP is associated with a series of allosteric conformational changes in CRM1 that lead to opening of the NES-binding cleft, allowing for stable binding of NES cargoes. A 2.1-A-resolution crystal structure of unliganded CRM1 reveals the mechanism of autoinhibition.,Saito N, Matsuura Y J Mol Biol. 2013 Jan 23;425(2):350-64. doi: 10.1016/j.jmb.2012.11.014. Epub 2012 , Nov 16. PMID:23164569 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Exportin

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