4hhg

Crystal structure of the Pseudomonas aeruginosa azurin, RuH107NO YOH109

spinqualitylabelsall models 4hhg, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hhg is a 1 chain structure with sequence from Pseudomonas aeruginosa pao1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU, DRU NonStd Res: NIY Gene: azu, PA4922 (Pseudomonas aeruginosa PAO1) Resources: FirstGlance, OCA, RCSB, PDBsum Function [AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase. Publication Abstract from PubMed We have designed ruthenium-modified Pseudomonas aeruginosa azurins that incorporate 3-nitrotyrosine (NO2YOH) between Ru(2,2'-bipyridine)2(imidazole)(histidine) and Cu redox centers in electron transfer (ET) pathways. We investigated the structures and reactivities of three different systems: RuH107NO2YOH109, RuH124NO2YOH122, and RuH126NO2YOH122. RuH107NO2YOH109, unlabeled H124NO2YOH122, and unlabeled H126NO2YOH122 were structurally characterized. The pKa's of NO2YOH at positions 122 and 109 are 7.2 and 6.0, respectively. Reduction potentials of 3-nitrotyrosinate (NO2YO-)-modified azurins were estimated from cyclic and differential pulse voltammetry data: oxidation of NO2YO-122 occurs near 1.1 versus NHE; oxidation of NO2YO-109 is near 1.2 V. Our analysis of transient optical spectroscopic experiments indicates that hopping via NO2YO- enhances CuI oxidation rates over single-step ET by factors of 32 (RuH107NO2YO-109), 46 (RuH126NO2YO-122), and 13 (RuH124NO2YO-122). Electron Flow through Nitrotyrosinate in Pseudomonas aeruginosa Azurin.,Warren JJ, Herrera N, Hill MG, Winkler JR, Gray HB J Am Chem Soc. 2013 Jul 16. PMID:23859602 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Azurin

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