4ane

R80N MUTANT OF NUCLEOSIDE DIPHOSPHATE KINASE FROM MYCOBACTERIUM TUBERCULOSIS

spinqualitylabelsall models 4ane, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ane is a 6 chain structure with sequence from "bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CIT Related: 1k44, 4anc, 4and Activity: Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Resources: FirstGlance, OCA, RCSB, PDBsum Function [NDK_MYCTU] Major role in the synthesis of nucleoside triphosphates other than ATP.[HAMAP-Rule:MF_00451] Publication Abstract from PubMed The crystal structure of the wild-type nucleoside diphosphate kinase from Mycobacterium tuberculosis at 2.6 A resolution revealed that the intersubunit salt bridge Arg80-Asp93 contributes to the thermal stability of the hexamer (Tm = 76 degrees C). On mutating Asp93 to Asn to break the salt bridge, the thermal stability dramatically decreased by 27.6 degrees C. Here, on mutating Arg80 to Asn, the thermal stability also significantly decreased by 8.0 degrees C. In the X-ray structure of the R80N mutant solved at 1.9 A resolution the salt bridge was replaced by intersubunit hydrogen bonds that contribute to the thermal stability of the hexamer. A citrate anion from the crystallization buffer was bound at the bottom of the nucleotide-binding site via electrostatic and hydrogen-bonding interactions with six conserved residues involved in nucleotide binding. Structural analysis shows that the citrate is present at the location of the nucleotide phosphate groups. Structure of Mycobacterium tuberculosis nucleoside diphosphate kinase R80N mutant in complex with citrate.,Georgescauld F, Moynie L, Habersetzer J, Dautant A Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):40-3. doi:, 10.1107/S2053230X13034134. Epub 2013 Dec 24. PMID:24419614 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoside diphosphate kinase

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