4kr1

Crystal structure of the kinetechore protein Iml3 from budding yeast

spinqualitylabelsall models 4kr1, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4kr1 is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Gene: IML3, MCM19, YBR107C, YBR0836 (Baker's yeast) Resources: FirstGlance, OCA, RCSB, PDBsum Function [IML3_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Publication Abstract from PubMed Human CENP-N and CENP-L have been reported to selectively recognize the CENP-A nucleosome and to contribute to recruiting other constitutive centromere-associated network (CCAN) complexes involved in assembly of the inner kinetochore. As their homologues, Chl4 and Iml3 from budding yeast function in a similar way in de novo assembly of the kinetochore. A lack of biochemical and structural information precludes further understanding of their exact role at the molecular level. Here, the crystal structure of Iml3 is presented and the structure shows that Iml3 adopts an elongated conformation with a series of intramolecular interactions. Pull-down assays revealed that the C-terminal domain of Chl4, which forms a dimer in solution, is responsible for Iml3 binding. Acting as a heterodimer, the Chl4-Iml3 complex exhibits a low-affinity nonspecific DNA-binding activity which may play an important role in the kinetochore-assembly process. Structural insights into the role of the Chl4-Iml3 complex in kinetochore assembly.,Guo Q, Tao Y, Liu H, Teng M, Li X Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2412-9. doi:, 10.1107/S0907444913022397. Epub 2013 Nov 19. PMID:24311582 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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