1ufp: Difference between revisions
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|PDB= 1ufp |SIZE=350|CAPTION= <scene name='initialview01'>1ufp</scene>, resolution 2.10Å | |PDB= 1ufp |SIZE=350|CAPTION= <scene name='initialview01'>1ufp</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1ufj|1UFJ]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ufp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufp OCA], [http://www.ebi.ac.uk/pdbsum/1ufp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ufp RCSB]</span> | |||
}} | }} | ||
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[[Category: Watanabe, Y.]] | [[Category: Watanabe, Y.]] | ||
[[Category: Yamane, T.]] | [[Category: Yamane, T.]] | ||
[[Category: iron]] | [[Category: iron]] | ||
[[Category: metalloprotein]] | [[Category: metalloprotein]] | ||
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[[Category: schiff base]] | [[Category: schiff base]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:09:39 2008'' | ||
Revision as of 21:09, 30 March 2008
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| 1ufp, resolution 2.10Å | |||||||||||||
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| Ligands: | PO4 | ||||||||||||
| Related: | 1UFJ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-wild type Myoglobin
Overview
Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.
About this Structure
1UFP is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.
Reference
Crystal structures of artificial metalloproteins: tight binding of FeIII(Schiff-Base) by mutation of Ala71 to Gly in apo-myoglobin., Ueno T, Ohashi M, Kono M, Kondo K, Suzuki A, Yamane T, Watanabe Y, Inorg Chem. 2004 May 3;43(9):2852-8. PMID:15106972
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