Sandbox Reserved 960: Difference between revisions

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== Ligands ==
== Ligands ==
In order to determine this {{Template:ColorKey Composition Protein}}’s structure, several {{Template:ColorKey Composition Ligand}} has been used at pH 5.5 because this low pH fits with its natural medium in the bee antenna.
[[Image:CMJ_Ligplot.png|150px|right|thumb|'''Fig.2''' CMJ Ligplot]]
[[Image:CMJ_Ligplot.png|150px|right|thumb|'''Fig.2''' CMJ Ligplot]]
In order to determine this {{Template:ColorKey Composition Protein}}’s structure, several {{Template:ColorKey Composition Ligand}} has been used at pH 5.5 because this low pH fits with its natural medium in the bee antenna.
The three ligands used to characterize and purify AmelASP1 are :
The three ligands used to characterize and purify AmelASP1 are :
*<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene>
*<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene>
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*<scene name='60/604479/Gol/1'>Glycerol</scene> (C3H8O3) also known as GOL, is a ligand used for cryoprotection during the purification process of the protein. [[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig.3''' GOL Ligplot]]It is supposedly helping the main ligand to reach its binding site. To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene>




 
*<scene name='60/604479/Cl/1'>Chloride ion</scene> facilitates the binding of other ligands to the protein. [[Image:Cl_Ligplot.png|right|thumb|'''Fig.4''' Cl Ligplot]]Its abundance around ASP1 varies according to changing pH conditions. At pH 5.5, <scene name='60/604479/Cl_binding_residue/1'>Val 65</scene> is the only amino acid able to fix a chloride ion.  
 
 
 
 
 
*<scene name='60/604479/Gol/1'>Glycerol</scene> (C3H8O3) also known as GOL, is a ligand used for cryoprotection during the purification process of the protein. It is supposedly helping the main ligand to reach its binding site. To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene>
[[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig.3''' GOL Ligplot]]
 
 
 
 
 
 
 
 
 
 
 
 
*<scene name='60/604479/Cl/1'>Chloride ion</scene> facilitates the binding of other ligands to the protein. Its abundance around ASP1 varies according to changing pH conditions. At pH 5.5, <scene name='60/604479/Cl_binding_residue/1'>Val 65</scene> is the only amino acid able to fix a chloride ion.  
[[Image:Cl_Ligplot.png|right|thumb|'''Fig.4''' Cl Ligplot]]


However, in natural conditions, binding ligands are the pheromones secreted by the queen such as 9-ODA.  
However, in natural conditions, binding ligands are the pheromones secreted by the queen such as 9-ODA.  
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