Sandbox Reserved 960: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 57: | Line 57: | ||
== Ligands == | == Ligands == | ||
[[Image:CMJ_Ligplot.png|150px|right|thumb|'''Fig. | [[Image:CMJ_Ligplot.png|150px|right|thumb|'''Fig.1''' CMJ Ligplot]] | ||
In order to determine this {{Template:ColorKey Composition Protein}}’s structure, several {{Template:ColorKey Composition Ligand}} has been used at pH 5.5 because this low pH fits with its natural medium in the bee antenna. | In order to determine this {{Template:ColorKey Composition Protein}}’s structure, several {{Template:ColorKey Composition Ligand}} has been used at pH 5.5 because this low pH fits with its natural medium in the bee antenna. | ||
| Line 63: | Line 63: | ||
*<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene> | *<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene> | ||
*[[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig. | *[[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig.2''' GOL Ligplot]]<scene name='60/604479/Gol/1'>Glycerol</scene> (C3H8O3) also known as GOL, is a ligand used for cryoprotection during the purification process of the protein. It is supposedly helping the main ligand to reach its binding site. | ||
To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene> | To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene> | ||
[[Image:Cl_Ligplot.png|right|thumb|'''Fig.3''' Cl Ligplot]] | |||
[[Image:Cl_Ligplot.png|right|thumb|'''Fig. | |||
