4pvz: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2==

== Structural highlights ==

<table><tr><td colspan='2'>[[4pvz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PVZ FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bk5|1bk5]], [[1ee4|1ee4]], [[1ee5|1ee5]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pvz RCSB], [http://www.ebi.ac.uk/pdbsum/4pvz PDBsum]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST]] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref>

== Publication Abstract from PubMed ==

Targeting of ER-synthesized membrane proteins to the inner nuclear membrane (INM) has long been explained by the diffusion-retention model. However, several INM proteins contain non-classical nuclear localization signal (NLS) sequences, which, in a few instances, have been shown to promote importin alpha/beta- and Ran-dependent translocation to the INM. Here, using structural and biochemical methods, we show that yeast INM proteins Heh2 and Src1/Heh1 contain bipartite import sequences that associate intimately with the minor NLS-binding pocket of yeast importin alpha and unlike classical NLSs efficiently displace the IBB domain in the absence of importin beta. In vivo, the intimate interactions at the minor NLS-binding pocket make the h2NLS highly efficient at recruiting importin alpha at the ER and drive INM localization of endogenous Heh2. Thus, h1/h2NLSs delineate a novel class of super-potent, IBB-like membrane protein NLSs, distinct from classical NLSs found in soluble cargos and of general interest in biology.

~~The entry 4pvz is ON HOLD until sometime in~~ the ~~future~~

Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.,Lokareddy RK, Hapsari RA, van Rheenen M, Pumroy RA, Bhardwaj A, Steen A, Veenhoff LM, Cingolani G Structure. 2015 May 19. pii: S0969-2126(15)00175-6. doi:, 10.1016/j.str.2015.04.017. PMID:26051712<ref>PMID:26051712</ref>

~~Authors: Lokareddy~~, R.K.~~, Hapsari, A.R., van Rheenen, M., Bhardwaj, A., Veenhoff, L.M., Cingolani, C~~.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description: Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2~~

== References ==

[[Category: ~~Unreleased Structures~~]]

__TOC__

</StructureSection>

[[Category: Bhardwaj, A]]

[[Category: Cingolani, C]]

[[Category: Lokareddy, R.K]]

[[Category: Hapsari, A R]]

[[Category: ~~Van~~ Rheenen, M]]

[[Category: Lokareddy, R K]]

[[Category: ~~Hapsari~~, ~~A.R~~]]

[[Category: Rheenen, M van]]

[[Category: ~~Bhardwaj, A~~]]

[[Category: Veenhoff, L M]]

[[Category: ~~Veenhoff, L.M~~]]

[[Category: Armadillo repeated structure]]

[[Category: Importin beta]]

[[Category: Nls-cargo]]

[[Category: Nuclear import adapter]]

[[Category: Protein binding]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2==
+<StructureSection load='4pvz' size='340' side='right' caption='[[4pvz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4pvz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PVZ FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bk5|1bk5]], [[1ee4|1ee4]], [[1ee5|1ee5]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pvz RCSB], [http://www.ebi.ac.uk/pdbsum/4pvz PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST]] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Targeting of ER-synthesized membrane proteins to the inner nuclear membrane (INM) has long been explained by the diffusion-retention model. However, several INM proteins contain non-classical nuclear localization signal (NLS) sequences, which, in a few instances, have been shown to promote importin alpha/beta- and Ran-dependent translocation to the INM. Here, using structural and biochemical methods, we show that yeast INM proteins Heh2 and Src1/Heh1 contain bipartite import sequences that associate intimately with the minor NLS-binding pocket of yeast importin alpha and unlike classical NLSs efficiently displace the IBB domain in the absence of importin beta. In vivo, the intimate interactions at the minor NLS-binding pocket make the h2NLS highly efficient at recruiting importin alpha at the ER and drive INM localization of endogenous Heh2. Thus, h1/h2NLSs delineate a novel class of super-potent, IBB-like membrane protein NLSs, distinct from classical NLSs found in soluble cargos and of general interest in biology.
-The entry 4pvz is ON HOLD  until sometime in the future
+Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.,Lokareddy RK, Hapsari RA, van Rheenen M, Pumroy RA, Bhardwaj A, Steen A, Veenhoff LM, Cingolani G Structure. 2015 May 19. pii: S0969-2126(15)00175-6. doi:, 10.1016/j.str.2015.04.017. PMID:26051712<ref>PMID:26051712</ref>
-Authors: Lokareddy, R.K., Hapsari, A.R., van Rheenen, M., Bhardwaj, A., Veenhoff, L.M., Cingolani, C.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bhardwaj, A]]
 [[Category: Cingolani, C]]
-[[Category: Lokareddy, R.K]]
+[[Category: Hapsari, A R]]
-[[Category: Van Rheenen, M]]
+[[Category: Lokareddy, R K]]
-[[Category: Hapsari, A.R]]
+[[Category: Rheenen, M van]]
-[[Category: Bhardwaj, A]]
+[[Category: Veenhoff, L M]]
-[[Category: Veenhoff, L.M]]
+[[Category: Armadillo repeated structure]]
+[[Category: Importin beta]]
+[[Category: Nls-cargo]]
+[[Category: Nuclear import adapter]]
+[[Category: Protein binding]]