4qfv: Difference between revisions
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''' | ==Crystal structure of a unique ankyrin== | ||
<StructureSection load='4qfv' size='340' side='right' caption='[[4qfv]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4qfv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QFV FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o60|4o60]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qfv RCSB], [http://www.ebi.ac.uk/pdbsum/4qfv PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibits the melibiose-dependent expression of alpha-galactosidase (MelA) and melibiose permease (MelB) of Escherichia coli by specifically preventing activation of the melAB operon. High-resolution crystal structural determination reveals that the designed ANK-N5C protein has a typical ankyrin fold, and the specific transcription blocker, ANK-N5C-281, forms a domain-swapped dimer. Functional tests suggest that the activity of MelR, a DNA-binding transcription activator and a member of AraC family of transcription factors, is inhibited by ANK-N5C-281 protein. All ANK-N5C proteins are expected to have a concave binding area with negative surface potential, suggesting that the designed ANK-N5C library proteins may facilitate the discovery of binders recognizing structural motifs with positive surface potential, like in DNA-binding proteins. Overall, our results show that the established library is a useful tool for the discovery of novel bioactive reagents. | |||
A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen.,Tikhonova EB, Ethayathulla AS, Su Y, Hariharan P, Xie S, Guan L Sci Rep. 2015 Jan 28;5:8070. doi: 10.1038/srep08070. PMID:25627011<ref>PMID:25627011</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
[[Category: Ethayathulla, A | __TOC__ | ||
</StructureSection> | |||
[[Category: Ethayathulla, A S]] | |||
[[Category: Guan, L]] | [[Category: Guan, L]] | ||
[[Category: Tikhonova, E | [[Category: Tikhonova, E B]] | ||
[[Category: De novo protein]] | |||
[[Category: Designed ankyrin repeat]] | |||
[[Category: Domain swap]] | |||
Revision as of 12:51, 6 May 2015
Crystal structure of a unique ankyrin
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