4rfr: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 4rfr ~~is ON HOLD until Paper Publication~~

==Complex structure of AlkB/rhein==

~~Authors~~: ~~Li, Q~~., ~~Huang~~, Y., Li, J.F., ~~Yang, C~~.-G.

== Structural highlights ==

<table><tr><td colspan='2'>[[4rfr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFR OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RFR FirstGlance].

~~Description~~: ~~Complex structure of AlkB~~/~~rhein~~

</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=RHN:4,5-DIHYDROXY-9,10-DIOXO-9,10-DIHYDROANTHRACENE-2-CARBOXYLIC+ACID'>RHN</scene></td></tr>

[[~~Category~~: ~~Unreleased Structures~~]]

<tr id='related'><td class="sblockLbl">[[Related_structure|Related:]]</td><td class="sblockDat">[[3t4h|3t4h]]</td></tr>

[[~~Category~~: ~~Li, J~~.F]]

<tr id='activity'><td class="sblockLbl">Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/DNA_oxidative_demethylase DNA oxidative demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.33 1.14.11.33] </td></tr>

[[Category: ~~Yang, C.-G~~]]

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfr OCA], [http://pdbe.org/4rfr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rfr RCSB], [http://www.ebi.ac.uk/pdbsum/4rfr PDBsum]</td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/ALKB_ECOLI ALKB_ECOLI]] Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).<ref>PMID:12226668</ref> <ref>PMID:12594517</ref> <ref>PMID:16482161</ref> <ref>PMID:19706517</ref> <ref>PMID:21068844</ref> <ref>PMID:20084272</ref>

== References ==

__TOC__

</StructureSection>

[[Category: DNA oxidative demethylase]]

[[Category: Huang, Y]]

[[Category: Li, J F]]

[[Category: Li, Q]]

[[Category: Yang, C G]]

[[Category: Alkb family]]

[[Category: Demethylase]]

[[Category: Demethylation]]

[[Category: Jellyroll fold]]

[[Category: Nucleic acid binding]]

[[Category: Nucleus]]

[[Category: Oxidoreductase-inhibitor complex]]

[[Category: Protein-inhibitor complex]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4rfr is ON HOLD  until Paper Publication
+==Complex structure of AlkB/rhein==
+<StructureSection load='4rfr' size='340' side='right' caption='[[4rfr]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-Authors: Li, Q., Huang, Y., Li, J.F., Yang, C.-G.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4rfr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RFR FirstGlance]. <br>
-Description: Complex structure of AlkB/rhein
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=RHN:4,5-DIHYDROXY-9,10-DIOXO-9,10-DIHYDROANTHRACENE-2-CARBOXYLIC+ACID'>RHN</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t4h|3t4h]]</td></tr>
-[[Category: Li, J.F]]
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_oxidative_demethylase DNA oxidative demethylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.33 1.14.11.33] </span></td></tr>
-[[Category: Yang, C.-G]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfr OCA], [http://pdbe.org/4rfr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rfr RCSB], [http://www.ebi.ac.uk/pdbsum/4rfr PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ALKB_ECOLI ALKB_ECOLI]] Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).<ref>PMID:12226668</ref> <ref>PMID:12594517</ref> <ref>PMID:16482161</ref> <ref>PMID:19706517</ref> <ref>PMID:21068844</ref> <ref>PMID:20084272</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: DNA oxidative demethylase]]
 [[Category: Huang, Y]]
+[[Category: Li, J F]]
 [[Category: Li, Q]]
+[[Category: Yang, C G]]
+[[Category: Alkb family]]
+[[Category: Demethylase]]
+[[Category: Demethylation]]
+[[Category: Jellyroll fold]]
+[[Category: Nucleic acid binding]]
+[[Category: Nucleus]]
+[[Category: Oxidoreductase-inhibitor complex]]
+[[Category: Protein-inhibitor complex]]