4rig: Difference between revisions
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''' | ==Chimeric Glycosyltransferase LanGT2S8Ac== | ||
<StructureSection load='4rig' size='340' side='right' caption='[[4rig]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4rig]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RIG FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rig OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rig RCSB], [http://www.ebi.ac.uk/pdbsum/4rig PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structures of the O-glycosyltransferase LanGT2 and the engineered, CC bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates. | |||
Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.,Tam HK, Harle J, Gerhardt S, Rohr J, Wang G, Thorson JS, Bigot A, Lutterbeck M, Seiche W, Breit B, Bechthold A, Einsle O Angew Chem Int Ed Engl. 2015 Jan 7. doi: 10.1002/anie.201409792. PMID:25581707<ref>PMID:25581707</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
[[Category: | </StructureSection> | ||
[[Category: Bechthold, A]] | |||
[[Category: Breit, B]] | [[Category: Breit, B]] | ||
[[Category: Einsle, O]] | [[Category: Einsle, O]] | ||
[[Category: Gerhardt, S]] | |||
[[Category: Tam, H K]] | |||
[[Category: C-glycosylating glycosyltransferase]] | |||
[[Category: Gt fold]] | |||
[[Category: Transferase]] | |||
Revision as of 16:33, 28 January 2015
Chimeric Glycosyltransferase LanGT2S8Ac
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