4x01: Difference between revisions

Revision as of 13:49, 14 January 2015

S. pombe Ctp1 tetramerization domain

Structural highlights

4x01 is a 8 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	EDO
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[COM1_SCHPO] Endonuclease that cooperates with the MRN complex in processing meiotic and mitotic double-strand breaks by allowing the endonucleolytic removal of rec12 from the break sites and ensuring both resection and intrachromosomal association of the broken ends. Required for the formation of RPA-coated single strand DNA adjacent to the DSBs where it functions together with the MRN complex in 5'- 3' resection. Required for the repair of programmed meiotic DSBs. Involved also in an rhp51 recombinase-dependent recombinational repair pathway.^[1] ^[2] ^[3] ^[4]

References

↑ Martin-Castellanos C, Blanco M, Rozalen AE, Perez-Hidalgo L, Garcia AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S. A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr Biol. 2005 Nov 22;15(22):2056-62. PMID:16303567 doi:https://dx.doi.org/S0960-9822(05)01277-7
↑ Limbo O, Chahwan C, Yamada Y, de Bruin RA, Wittenberg C, Russell P. Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination. Mol Cell. 2007 Oct 12;28(1):134-46. PMID:17936710 doi:https://dx.doi.org/10.1016/j.molcel.2007.09.009
↑ Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H. Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex. Mol Cell Biol. 2008 Jun;28(11):3639-51. doi: 10.1128/MCB.01828-07. Epub 2008 Mar , 31. PMID:18378696 doi:https://dx.doi.org/10.1128/MCB.01828-07
↑ Hartsuiker E, Mizuno K, Molnar M, Kohli J, Ohta K, Carr AM. Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions. Mol Cell Biol. 2009 Apr;29(7):1671-81. doi: 10.1128/MCB.01182-08. Epub 2009 Jan, 12. PMID:19139281 doi:https://dx.doi.org/10.1128/MCB.01182-08

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OCA

[1] Martin-Castellanos C, Blanco M, Rozalen AE, Perez-Hidalgo L, Garcia AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S. A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr Biol. 2005 Nov 22;15(22):2056-62. PMID:16303567 doi:https://dx.doi.org/S0960-9822(05)01277-7

[2] Limbo O, Chahwan C, Yamada Y, de Bruin RA, Wittenberg C, Russell P. Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination. Mol Cell. 2007 Oct 12;28(1):134-46. PMID:17936710 doi:https://dx.doi.org/10.1016/j.molcel.2007.09.009

[3] Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, Iwasaki H. Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex. Mol Cell Biol. 2008 Jun;28(11):3639-51. doi: 10.1128/MCB.01828-07. Epub 2008 Mar , 31. PMID:18378696 doi:https://dx.doi.org/10.1128/MCB.01828-07

[4] Hartsuiker E, Mizuno K, Molnar M, Kohli J, Ohta K, Carr AM. Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions. Mol Cell Biol. 2009 Apr;29(7):1671-81. doi: 10.1128/MCB.01182-08. Epub 2009 Jan, 12. PMID:19139281 doi:https://dx.doi.org/10.1128/MCB.01182-08

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==S. pombe Ctp1 tetramerization domain==
+<StructureSection load='4x01' size='340' side='right' caption='[[4x01]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-The entry 4x01 is ON HOLD  until Paper Publication
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4x01]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X01 FirstGlance]. <br>
-Authors: Andres, S.N., Williams, R.S.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x01 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x01 RCSB], [http://www.ebi.ac.uk/pdbsum/4x01 PDBsum]</span></td></tr>
-Description:
+</table>
-[[Category: Unreleased Structures]]
+== Function ==
-[[Category: Andres, S.N]]
+[[http://www.uniprot.org/uniprot/COM1_SCHPO COM1_SCHPO]] Endonuclease that cooperates with the MRN complex in processing meiotic and mitotic double-strand breaks by allowing the endonucleolytic removal of rec12 from the break sites and ensuring both resection and intrachromosomal association of the broken ends. Required for the formation of RPA-coated single strand DNA adjacent to the DSBs where it functions together with the MRN complex in 5'- 3' resection. Required for the repair of programmed meiotic DSBs. Involved also in an rhp51 recombinase-dependent recombinational repair pathway.<ref>PMID:16303567</ref> <ref>PMID:17936710</ref> <ref>PMID:18378696</ref> <ref>PMID:19139281</ref>
-[[Category: Williams, R.S]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Andres, S N]]
+[[Category: Williams, R S]]
+[[Category: Dna binding]]
+[[Category: Dna double-strand break repair]]
+[[Category: Four-helix bundle]]
+[[Category: Homologous recombination]]

4x01: Difference between revisions

Revision as of 13:49, 14 January 2015

S. pombe Ctp1 tetramerization domain

Structural highlights

Function

References

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