4x2h: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Sac3N peptide bound to Mex67:Mtr2==

== Structural highlights ==

<table><tr><td colspan='2'>[[4x2h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X2H FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x2h RCSB], [http://www.ebi.ac.uk/pdbsum/4x2h PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.

~~The entry 4x2h is ON HOLD~~

Structural Characterization of the Chaetomium thermophilum TREX-2 Complex and its Interaction with the mRNA Nuclear Export Factor Mex67:Mtr2.,Dimitrova L, Valkov E, Aibara S, Flemming D, McLaughlin SH, Hurt E, Stewart M Structure. 2015 Jun 3. pii: S0969-2126(15)00180-X. doi:, 10.1016/j.str.2015.05.002. PMID:26051714<ref>PMID:26051714</ref>

~~Authors: Aibara~~, S.~~, Valkov, E., Stewart, M~~.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description: Sac3N peptide bound to Mex67:Mtr2~~

== References ==

~~[[Category: Unreleased Structures]]~~

__TOC__

</StructureSection>

[[Category: Aibara, S]]

[[Category: Stewart, M]]

[[Category: Valkov, E]]

[[Category: ~~Stewart, M~~]]

[[Category: Mrna nuclear export]]

[[Category: Transcription]]

[[Category: Trex-2]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Sac3N peptide bound to Mex67:Mtr2==
+<StructureSection load='4x2h' size='340' side='right' caption='[[4x2h]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4x2h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X2H FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x2h RCSB], [http://www.ebi.ac.uk/pdbsum/4x2h PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.
-The entry 4x2h is ON HOLD
+Structural Characterization of the Chaetomium thermophilum TREX-2 Complex and its Interaction with the mRNA Nuclear Export Factor Mex67:Mtr2.,Dimitrova L, Valkov E, Aibara S, Flemming D, McLaughlin SH, Hurt E, Stewart M Structure. 2015 Jun 3. pii: S0969-2126(15)00180-X. doi:, 10.1016/j.str.2015.05.002. PMID:26051714<ref>PMID:26051714</ref>
-Authors: Aibara, S., Valkov, E., Stewart, M.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Sac3N peptide bound to Mex67:Mtr2
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Aibara, S]]
+[[Category: Stewart, M]]
 [[Category: Valkov, E]]
-[[Category: Stewart, M]]
+[[Category: Mrna nuclear export]]
+[[Category: Transcription]]
+[[Category: Trex-2]]