4r5f

X-ray structure of the D199K mutant of the cysteine desulfurase IscS from A. fulgidus

spinqualitylabelsall models 4r5f, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4r5f is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, CA, CL, PG4, PMP Related: 4hvk, 4eb5, 4eb7 Activity: Cysteine desulfurase, with EC number 2.8.1.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). Publication Abstract from PubMed Iron sulfur ([Fe-S]) clusters are essential prosthetic groups involved in fundamental cell processes such as gene expression regulation, electron transfer and Lewis acid base chemistry. Central components of their biogenesis are pyridoxal-5'-phosphate (PLP) dependent l-cysteine desulfurases, which provide the necessary S atoms for [Fe-S] cluster assembly. The archaeon Archaeoglobus fulgidus (Af) has two ORFs, which although annotated as l-cysteine desulfurases of the ISC type (IscS), lack the essential Lys residue (K199 in Af) that forms a Schiff base with PLP. We have previously determined the structure of an Af(IscU-D35A-IscS)2 complex heterologously expressed in Escherichia coli and found it to contain a [Fe2S2] cluster. In order to understand the origin of sulfide in that structure we have performed a series of functional tests using wild type and mutated forms of AfIscS. In addition, we have determined the crystal structure of an AfIscS-D199K mutant. From these studies we conclude that: i) AfIscS has no desulfurase activity; ii) in our in vitro [Fe2S2] cluster assembly experiments, sulfide ions are non-enzymatically generated by a mixture of iron, l-cysteine and PLP and iii) the physiological role of AfIscS may be to provide a cysteine ligand to the nascent cluster as observed in the [Fe2S2]-Af(IscU-D35A-IscS)2 complex. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases. IscS from Archaeoglobus fulgidus has no desulfurase activity but may provide a cysteine ligand for [FeS] cluster assembly.,Pagnier A, Nicolet Y, Fontecilla-Camps JC Biochim Biophys Acta. 2014 Oct 28. pii: S0167-4889(14)00374-7. doi:, 10.1016/j.bbamcr.2014.10.015. PMID:25447670 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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