4ljy: Difference between revisions

Revision as of 02:10, 5 August 2016

Crystal structure of RNA splicing effector Prp5 in complex with ADP

Structural highlights

4ljy is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ADP, MG, MRD
NonStd Res:	MSE
Related:	4lk2
Gene:	PRP5, RNA5, YBR237W, YBR1603 (Baker's yeast)
Activity:	RNA helicase, with EC number 3.6.4.13
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PRP5_YEAST] ATP-dependent RNA helicase involved spliceosome assembly and in nuclear splicing. Catalyzes an ATP-dependent conformational change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

References

↑ Ruby SW, Chang TH, Abelson J. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev. 1993 Oct;7(10):1909-25. PMID:8405998
↑ Wells SE, Ares M Jr. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Mol Cell Biol. 1994 Sep;14(9):6337-49. PMID:8065365
↑ O'Day CL, Dalbadie-McFarland G, Abelson J. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. J Biol Chem. 1996 Dec 27;271(52):33261-7. PMID:8969184
↑ Wiest DK, O'Day CL, Abelson J. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem. 1996 Dec 27;271(52):33268-76. PMID:8969185
↑ Yan D, Ares M Jr. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol. 1996 Mar;16(3):818-28. PMID:8622683
↑ Abu Dayyeh BK, Quan TK, Castro M, Ruby SW. Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. J Biol Chem. 2002 Jun 7;277(23):20221-33. Epub 2002 Apr 1. PMID:11927574 doi:https://dx.doi.org/10.1074/jbc.M109553200
↑ Perriman R, Barta I, Voeltz GK, Abelson J, Ares M Jr. ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13857-62. Epub 2003 Nov 10. PMID:14610285 doi:https://dx.doi.org/10.1073/pnas.2036312100
↑ Wang Q, He J, Lynn B, Rymond BC. Interactions of the yeast SF3b splicing factor. Mol Cell Biol. 2005 Dec;25(24):10745-54. PMID:16314500 doi:https://dx.doi.org/25/24/10745

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OCA

[1] Ruby SW, Chang TH, Abelson J. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev. 1993 Oct;7(10):1909-25. PMID:8405998

[2] Wells SE, Ares M Jr. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Mol Cell Biol. 1994 Sep;14(9):6337-49. PMID:8065365

[3] O'Day CL, Dalbadie-McFarland G, Abelson J. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. J Biol Chem. 1996 Dec 27;271(52):33261-7. PMID:8969184

[4] Wiest DK, O'Day CL, Abelson J. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem. 1996 Dec 27;271(52):33268-76. PMID:8969185

[5] Yan D, Ares M Jr. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol. 1996 Mar;16(3):818-28. PMID:8622683

[6] Abu Dayyeh BK, Quan TK, Castro M, Ruby SW. Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. J Biol Chem. 2002 Jun 7;277(23):20221-33. Epub 2002 Apr 1. PMID:11927574 doi:https://dx.doi.org/10.1074/jbc.M109553200

[7] Perriman R, Barta I, Voeltz GK, Abelson J, Ares M Jr. ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13857-62. Epub 2003 Nov 10. PMID:14610285 doi:https://dx.doi.org/10.1073/pnas.2036312100

[8] Wang Q, He J, Lynn B, Rymond BC. Interactions of the yeast SF3b splicing factor. Mol Cell Biol. 2005 Dec;25(24):10745-54. PMID:16314500 doi:https://dx.doi.org/25/24/10745

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@@ Line 1: / Line 1: @@
 ==Crystal structure of RNA splicing effector Prp5 in complex with ADP==
 <StructureSection load='4ljy' size='340' side='right' caption='[[4ljy]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 8: / Line 9: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRP5, RNA5, YBR237W, YBR1603 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ljy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ljy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ljy RCSB], [http://www.ebi.ac.uk/pdbsum/4ljy PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ljy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ljy OCA], [http://pdbe.org/4ljy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ljy RCSB], [http://www.ebi.ac.uk/pdbsum/4ljy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ljy ProSAT]</span></td></tr>
 </table>
 == Function ==

4ljy: Difference between revisions

Revision as of 02:10, 5 August 2016

Crystal structure of RNA splicing effector Prp5 in complex with ADP

Structural highlights

Function

References

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