2mex

Structure of the tetrameric building block of the Salmonella Typhimurium PrgI Type three secretion system needle

spinqualitylabelsall models 2mex, 15 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mex is a 4 chain structure with sequence from Salty. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2lpz Gene: prgI, STM2873 (SALTY) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [PRGI_SALTY] Required for invasion of epithelial cells. Publication Abstract from PubMed Noncovalent supramolecular assemblies possess in general several unique subunit-subunit interfaces.The basic building block of such an assembly consists of several subunits and contains all unique interfaces. Atomic-resolution structures of monomeric subunits are typically accessed by crystallography or solution NMR and fitted into electron microscopy density maps. However, the structure of the intact building block in the assembled state remains unknown with this hybrid approach. Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. The building block structure consists of a homotetrameric subunit complex with three unique supramolecular interfaces. Side-chain positions at the interfaces were solved at atomic detail. The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements. Atomic structure and handedness of the building block of a biological assembly.,Loquet A, Habenstein B, Chevelkov V, Vasa SK, Giller K, Becker S, Lange A J Am Chem Soc. 2013 Dec 26;135(51):19135-8. doi: 10.1021/ja411362q. Epub 2013 Dec, 17. PMID:24328247 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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