4q10

The catalytic core of Rad2 in complex with DNA substrate (complex IV)

spinqualitylabelsall models 4q10, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4q10 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, K Related: 4q0w, 4q0z Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RAD2_YEAST] Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair. Publication Abstract from PubMed Rad2/XPG belongs to the flap nuclease family and is responsible for a key step of the eukaryotic nucleotide excision DNA repair (NER) pathway. To elucidate the mechanism of DNA binding by Rad2/XPG, we solved crystal structures of the catalytic core of Rad2 in complex with a substrate. Rad2 utilizes three structural modules for recognition of the double-stranded portion of DNA substrate, particularly a Rad2-specific alpha-helix for binding the cleaved strand. The protein does not specifically recognize the single-stranded portion of the nucleic acid. Our data suggest that in contrast to related enzymes (FEN1 and EXO1), the Rad2 active site may be more accessible, which would create an exit route for substrates without a free 5' end. Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding.,Mietus M, Nowak E, Jaciuk M, Kustosz P, Studnicka J, Nowotny M Nucleic Acids Res. 2014 Aug 12. pii: gku729. PMID:25120270 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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