2c5b: Difference between revisions
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|PDB= 2c5b |SIZE=350|CAPTION= <scene name='initialview01'>2c5b</scene>, resolution 2.50Å | |PDB= 2c5b |SIZE=350|CAPTION= <scene name='initialview01'>2c5b</scene>, resolution 2.50Å | ||
|SITE= <scene name='pdbsite=AC1:MET+Binding+Site+For+Chain+C'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:MET+Binding+Site+For+Chain+C'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=MET:METHIONINE'>MET</scene> and <scene name='pdbligand=5F1:5 | |LIGAND= <scene name='pdbligand=MET:METHIONINE'>MET</scene> and <scene name='pdbligand=5F1:5'-FLUORO-2',5'-DIDEOXYADENOSINE'>5F1</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] | ||
|GENE= | |GENE= | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:50:23 2008'' | ||
Revision as of 12:50, 23 March 2008
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| 2c5b, resolution 2.50Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Ligands: | MET and 5F1 | ||||||||||||
| Activity: | Adenosyl-fluoride synthase, with EC number 2.5.1.63 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
About this Structure
2C5B is a Single protein structure of sequence from Streptomyces cattleya. Full crystallographic information is available from OCA.
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
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