3rp6: Difference between revisions

Revision as of 16:59, 4 August 2016

Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD

Structural highlights

3rp6 is a 1 chain structure with sequence from Klep7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	FAD
NonStd Res:	MSE
Related:	3rp7, 3rp8
Gene:	KPN78578_16330, KPN_01663 (KLEP7)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HPXO_KLEP7] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.^[1]

Publication Abstract from PubMed

HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 A and 2.2 A, respectively. We have also determined the structure of the R204Q mutant at 2.0 A resolution in the absence of uric acid. The mutant structure is very similar to wild type HpxO except for the conformation of Arg103, which interacts with FAD in the mutant but not in the wild type structure. Interestingly, the R204Q mutation results in the uncoupling of NADH oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid activating it for the oxygen transfer. Based on these data, a mechanism for this reaction is proposed consisting of a nucleophilic attack of the urate anion on the flavin hydroperoxide resulting in the formation of 5-hydroxyisourate.

Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae.,Hicks KA, O'Leary SE, Begley TP, Ealick SE Biochemistry. 2012 Dec 21. PMID:23259842^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ O'Leary SE, Hicks KA, Ealick SE, Begley TP. Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase. Biochemistry. 2009 Apr 14;48(14):3033-5. doi: 10.1021/bi900160b. PMID:19260710 doi:https://dx.doi.org/10.1021/bi900160b
↑ Hicks KA, O'Leary SE, Begley TP, Ealick SE. Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae. Biochemistry. 2012 Dec 21. PMID:23259842 doi:https://dx.doi.org/10.1021/bi301262p

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OCA

[1] O'Leary SE, Hicks KA, Ealick SE, Begley TP. Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase. Biochemistry. 2009 Apr 14;48(14):3033-5. doi: 10.1021/bi900160b. PMID:19260710 doi:https://dx.doi.org/10.1021/bi900160b

[2] Hicks KA, O'Leary SE, Begley TP, Ealick SE. Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae. Biochemistry. 2012 Dec 21. PMID:23259842 doi:https://dx.doi.org/10.1021/bi301262p

[1]

[2]

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD==
 <StructureSection load='3rp6' size='340' side='right' caption='[[3rp6]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3rp6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_mgh_78578 Klebsiella pneumoniae subsp. pneumoniae mgh 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RP6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3rp6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RP6 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rp7|3rp7]], [[3rp8|3rp8]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 Klebsiella pneumoniae subsp. pneumoniae MGH 78578])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rp6 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp6 OCA], [http://pdbe.org/3rp6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rp6 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/A6T923_KLEP7 A6T923_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
+[[http://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3rp6" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Klebsiella pneumoniae subsp. pneumoniae mgh 78578]]
+[[Category: Klep7]]
 [[Category: Begley, T P]]
 [[Category: Ealick, S E]]

3rp6: Difference between revisions

Revision as of 16:59, 4 August 2016

Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD

Structural highlights

Function

Publication Abstract from PubMed

References

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