2d52: Difference between revisions

Revision as of 05:52, 11 September 2015

Pentaketide chromone synthase (M207G mutant complexed with Coa)

Structural highlights

2d52 is a 2 chain structure with sequence from Aloar. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	COA
NonStd Res:	CSD
Related:	2d3m, 1bq6, 1ee0, 2d51
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[PCS_ALOAR] Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structures of a wild-type and a mutant PCS, a novel plant type III polyketide synthase from a medicinal plant, Aloe arborescens, were solved at 1.6 A resolution. The crystal structures revealed that the pentaketide-producing wild-type and the octaketide-producing M207G mutant shared almost the same overall folding, and that the large-to-small substitution dramatically increases the volume of the polyketide-elongation tunnel by opening a gate to two hidden pockets behind the active site of the enzyme. The chemically inert active site residue 207 thus controls the number of condensations of malonyl-CoA, solely depending on the steric bulk of the side chain. These findings not only provided insight into the polyketide formation reaction, but they also suggested strategies for the engineered biosynthesis of polyketides.

Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens.,Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abe I, Utsumi Y, Oguro S, Morita H, Sano Y, Noguchi H. A plant type III polyketide synthase that produces pentaketide chromone. J Am Chem Soc. 2005 Feb 9;127(5):1362-3. PMID:15686354 doi:https://dx.doi.org/10.1021/ja0431206
↑ Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T. Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens. Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571 doi:https://dx.doi.org/10.1016/j.chembiol.2007.02.003

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OCA

[1] Abe I, Utsumi Y, Oguro S, Morita H, Sano Y, Noguchi H. A plant type III polyketide synthase that produces pentaketide chromone. J Am Chem Soc. 2005 Feb 9;127(5):1362-3. PMID:15686354 doi:https://dx.doi.org/10.1021/ja0431206

[2] Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T. Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens. Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571 doi:https://dx.doi.org/10.1016/j.chembiol.2007.02.003

[1]

[2]

@@ Line 2: / Line 2: @@
 <StructureSection load='2d52' size='340' side='right' caption='[[2d52]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2d52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aloe_arborescens Aloe arborescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D52 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2d52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aloar Aloar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D52 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d3m|2d3m]], [[1bq6|1bq6]], [[1ee0|1ee0]], [[2d51|2d51]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d52 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d52 RCSB], [http://www.ebi.ac.uk/pdbsum/2d52 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d52 OCA], [http://pdbe.org/2d52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d52 RCSB], [http://www.ebi.ac.uk/pdbsum/2d52 PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q58VP7_ALOAR Q58VP7_ALOAR]] Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.<ref>PMID:15686354</ref>
+[[http://www.uniprot.org/uniprot/PCS_ALOAR PCS_ALOAR]] Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.<ref>PMID:15686354</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 28: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2d52" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Aloe arborescens]]
+[[Category: Aloar]]
 [[Category: Kohno, T]]
 [[Category: Morita, H]]

2d52: Difference between revisions

Revision as of 05:52, 11 September 2015

Pentaketide chromone synthase (M207G mutant complexed with Coa)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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