2de6: Difference between revisions
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|PDB= 2de6 |SIZE=350|CAPTION= <scene name='initialview01'>2de6</scene>, resolution 1.80Å | |PDB= 2de6 |SIZE=350|CAPTION= <scene name='initialview01'>2de6</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2de5|2DE5]], [[2de7|2DE7]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2de6 OCA], [http://www.ebi.ac.uk/pdbsum/2de6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2de6 RCSB]</span> | |||
}} | }} | ||
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[[Category: Ashikawa, Y.]] | [[Category: Ashikawa, Y.]] | ||
[[Category: Nojiri, H.]] | [[Category: Nojiri, H.]] | ||
[[Category: | [[Category: carbazole 1,9a-dioxygenase]] | ||
[[Category: electron transfer complex]] | [[Category: electron transfer complex]] | ||
[[Category: rieske non-heme iron oxygenase system]] | [[Category: rieske non-heme iron oxygenase system]] | ||
| Line 34: | Line 34: | ||
[[Category: terminal oxygenase]] | [[Category: terminal oxygenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:33:32 2008'' | ||
Revision as of 23:33, 30 March 2008
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| 2de6, resolution 1.80Å | |||||||||||||
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| Ligands: | FE2, FES | ||||||||||||
| Related: | 2DE5, 2DE7
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase
Overview
Carbazole 1,9a-dioxygenase (CARDO), a member of the Rieske nonheme iron oxygenase system (ROS), consists of a terminal oxygenase (CARDO-O) and electron transfer components (ferredoxin [CARDO-F] and ferredoxin reductase [CARDO-R]). We determined the crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of CARDO-O with its electron donor, CARDO-F, at 1.9, 1.8, and 2.0 A resolutions, respectively. These structures provide the first structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in ROS. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole resulted in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site.
About this Structure
2DE6 is a Protein complex structure of sequences from Janthinobacterium and Pseudomonas resinovorans. Full crystallographic information is available from OCA.
Reference
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system., Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H, Structure. 2006 Dec;14(12):1779-89. PMID:17161368
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