Practical Guide to Homology Modeling: Difference between revisions
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You don’t need a homology model if the amino acid sequence of interest (the query sequence) already has an empirically determined 3D structure. Structures determined empirically, by X-ray crystallography or (much less often) by solution NMR, will almost always be more accurate than a homology model. | You don’t need a homology model if the amino acid sequence of interest (the query sequence) already has an empirically determined 3D structure. Structures determined empirically, by X-ray crystallography or (much less often) by solution NMR, will almost always be more accurate than a homology model. | ||
=== Is there an empirical model? === | |||
All published, empirically-determined, atomic-resolution, macromolecular 3D structures are available in the [[[Protein Data Bank]] (PDB, pdb.org). | |||
Each model in the PDB has a unique 4-character identification code ([[PDB ID]]) that begins with a numeral, and has letters or numerals for the last 3 characters . Examples are 1d66, 4mdh, 9ins. | |||
Here are two methods for finding out if your query amino acid sequence, or parts of it, have empirically-determined 3D structures in the PDB. | |||
== References == | == References == | ||
<references/> | <references/> | ||
