Practical Guide to Homology Modeling: Difference between revisions
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*Partially folded: The tumor suppressor protein p53 (length 393; e.g. [http://mobidb.bio.unipd.it/entries/P04637 P04637, P53_HUMAN]) is intrinsically disordered at both the N and C termini. There are many crystallographic structures for the folded mid-region (~200 residues), which lack coordinates for 90-some residues at the N terminus, and 90-some at the C terminus. Some solution NMR structures of the N terminus illustrate the disorder (e.g. 2ly4). | *Partially folded: The tumor suppressor protein p53 (length 393; e.g. [http://mobidb.bio.unipd.it/entries/P04637 P04637, P53_HUMAN]) is intrinsically disordered at both the N and C termini. There are many crystallographic structures for the folded mid-region (~200 residues), which lack coordinates for 90-some residues at the N terminus, and 90-some at the C terminus. Some solution NMR structures of the N terminus illustrate the disorder (e.g. 2ly4). | ||
*Unfolded: Caldesmon from chicken gizzard (length 771; [http://mobidb.bio.unipd.it/entries/P12957 P12957, CALD1_CHICK]) has no crystal structures, and is predicted to be disordered for essentially its full length. | *Unfolded: Caldesmon from chicken gizzard (length 771; [http://mobidb.bio.unipd.it/entries/P12957 P12957, CALD1_CHICK]) has no crystal structures, and is predicted to be disordered for essentially its full length. | ||
=== Prediction of intrinsic disorder === | |||
==== MobiDB via UniProt ==== | |||
At UniProt.Org, find your protein, then click on “Structure”. At the bottom of this section is usually a link to MobiDB’s report for the query protein. There, in the section ''Detailed Disorder Annotations'' are graphics showing experimental evidence for disorder (if available) and, under the heading ''Predictors'', results from several servers designed to predict intrinsic disorder. | |||
The ''Examples'' above are linked to MobiDB. | |||
==== FoldIndex ==== | |||
The [http://bip.weizmann.ac.il/fldbin/findex FoldIndex server] is a useful adjunct to the MobiDB report, since it is not included in that report. | |||
== References == | == References == | ||
<references/> | <references/> | ||
