Practical Guide to Homology Modeling: Difference between revisions

From Proteopedia
Jump to navigationJump to search
Eric Martz (talk | contribs)
Eric Martz (talk | contribs)
Line 161: Line 161:


To download a model, open the pull-down menu and pick ''Coordinates''.
To download a model, open the pull-down menu and pick ''Coordinates''.
===Generating New Models===
*At [http://uniprot.org UniProt.Org], find your sequence, and copy it in FASTA format.
*Go to [http://swissmodel.expasy.org SwissModel.expasy.org].
*It is a good idea to create an account, and login. This makes it easy to find your models later, although they are not kept on the server more than a week.
*Open the menu ''Modelling'' at the top, and select '''Automated Mode'''.
*Paste your sequence into the box, give the project a title, and click ''Build Model''. Processing can take from a few minutes to a few hours.
The results will have a table listing percentages of sequence identity, and templates used. Below will be molecular images for the models. Click on a molecular image to open more information. In the box that opens, click the symbol at right that looks like "'''v'''" to open more details.
To download a model, click on the blue button ''Model 01'' (or 02, 03, etc.) and pick PDB File.


==How To Explore 3D Models==
==How To Explore 3D Models==