RiAFP

spinqualitylabelsall models Insect antifreeze protein complex with sulfate and glycerol (PDB code 4dt5). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D The asymmetric unit comprises two RiAFP molecules juxtaposed with their ice-binding surfaces, however the protein is monomer in the solutio Overall Structure The crystallographic structure of RiAFP was defined recently. It reveals a new β-solenoid architecture that forms β-sandwich of two parallel 6 and 7 stranded-sheets of remarkable regularity. The β-sheets lie on top of each other with the upper and lower strands parallel but in the opposite orientation. Two ends deviate from β helix regularity by forming capping structures. These capping structures help to prevent end-to-end associations that would spoil the solubility of RiAFP and lead to oligomerization and aggregation. The three residues in β-strand 11 at the C terminus (Gln110, Gln112, and Ile114) that are too bulky to be accommodated into the core may also contribute to the capping structure to prevent amyloid-like polymerization. RiAFP solenoid possesses compressed nature. In the core of RiAFP, the side chains within apposed β-strands from the two β-sheets are staggered, allowing the side chains to interdigitate and pack tightly against one another. Most of the side chains in the core are from Ala, Ser and Thr. Those residues create a more compact fold that may contribute to the high stability and antifreeze activity of RiAFP. Within the core there are hydrogen bonds between Thr-Ser (65-55, 85-75, 132-124 respectively) and one disulfide bond between Cys4-Cys21, that contributes to stabilization of the whole structure. The β-turns in the structure contain mostly Gly or Pro residues. Function TextToBeDisplayed chain A without His-Tag Structural highlights This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

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Vera Sirotinskaya, Hila Cohen, Angel Herraez, Michal Harel