Sandbox Reserved 962: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 16: | Line 16: | ||
=== Cap Analog Binding (7-Methylguanosine-5-Triphosphate-5-Guanosine)=== | === Cap Analog Binding (7-Methylguanosine-5-Triphosphate-5-Guanosine)=== | ||
[[Image: | [[Image:M7GpppG.png]] | ||
<scene name='60/604481/Gtg/3'>A cap analog</scene> binds to the enzyme in a pocket near the AdoHcy. | <scene name='60/604481/Gtg/3'>A cap analog</scene> binds to the enzyme in a pocket near the AdoHcy. | ||
| Line 22: | Line 22: | ||
The cap makes Van der Walls contacts, hydrogen bond and water mediated bond. | The cap makes Van der Walls contacts, hydrogen bond and water mediated bond. | ||
<ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | <ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | ||
[[Image:Image_methyl_acceptor_specificity.png]] | |||
As we can see on the figure, the enzyme specifically binds to guanine. This specificity is achieved through the recognition by the enzyme residues through hydrogen bonds of the guanine N1, N3, and O6 atoms. We remark also that the methyltransferase is not able to discrminate between ribose and deoxyribose nucleoside sugars. | As we can see on the figure, the enzyme specifically binds to guanine. This specificity is achieved through the recognition by the enzyme residues through hydrogen bonds of the guanine N1, N3, and O6 atoms. We remark also that the methyltransferase is not able to discrminate between ribose and deoxyribose nucleoside sugars. | ||
=== AdoHcy Binding (S-Adenosyl-L-Homocysteine)=== | === AdoHcy Binding (S-Adenosyl-L-Homocysteine)=== | ||
[[Image:AdoHcy_nouveau.PNG]] | [[Image:AdoHcy_nouveau.PNG]] | ||
