Sandbox Reserved 962: Difference between revisions
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[[Image:Image_methyl_acceptor_specificity.png]] | [[Image:Image_methyl_acceptor_specificity.png]] | ||
{{clear}} | {{clear}} | ||
As we can see on the figure<ref name="Hausmann S, Zheng S, Fabrega C, Schneller SW, Lima CD, Shuman S. Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase: methyl acceptor specificity, inhibition BY S-adenosylmethionine analogs, and structure-guided mutational analysis. J Biol Chem. 2005 May 27;280(21):20404-12. Epub 2005 Mar 9.">PMID:15760890 </ref> , the enzyme specifically binds to guanine.{{clear}} | As we can see on the figure above<ref name="Hausmann S, Zheng S, Fabrega C, Schneller SW, Lima CD, Shuman S. Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase: methyl acceptor specificity, inhibition BY S-adenosylmethionine analogs, and structure-guided mutational analysis. J Biol Chem. 2005 May 27;280(21):20404-12. Epub 2005 Mar 9.">PMID:15760890 </ref> , the enzyme specifically binds to guanine.{{clear}} | ||
This specificity is achieved through the recognition by the enzyme residues through hydrogen bonds of the guanine N1, N3, and O6 atoms.{{clear}} | This specificity is achieved through the recognition by the enzyme residues through hydrogen bonds of the guanine N1, N3, and O6 atoms.{{clear}} | ||
We remark also that the methyltransferase is not able to discrminate between ribose and deoxyribose nucleoside sugars. | We remark also that the methyltransferase is not able to discrminate between ribose and deoxyribose nucleoside sugars. | ||
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- An electrostatic interaction mediated by Gln140, Phe141{{clear}} | - An electrostatic interaction mediated by Gln140, Phe141{{clear}} | ||
- A water mediated contact mediated by Asp78. | - A water mediated contact mediated by Asp78. | ||
<ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | <ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | ||
