Sandbox Reserved 960: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 61: | Line 61: | ||
The three ligands used to characterize and purify AmelASP1 are : | The three ligands used to characterize and purify AmelASP1 are : | ||
*<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene> | *<scene name='60/604479/Cmj/3'>CMJ</scene> also known as (20s)-20-Methyldotetracontane, is a serendipitous ligand. This term signify that the purification of this molecule was completely fortuitous. It is a big unsaturated mono-methyl branched carbone chain with formula C43H88. This ligand fits in the {{Template:ColorKey_Hydrophobic}} cavity of AmelASP1 thanks to several interactions with <scene name='60/604479/Cmj_binding_residues/2'>specific residues.</scene> ('''Fig.1''') | ||
*[[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig.2''' GOL Ligplot]]<scene name='60/604479/Gol/1'>Glycerol</scene> (C3H8O3) also known as GOL, is a ligand used for cryoprotection during the purification process of the protein. It is supposedly helping the main ligand to reach its binding site. | *[[Image:GOL_Ligplot.png|200px|left|thumb|'''Fig.2''' GOL Ligplot]]<scene name='60/604479/Gol/1'>Glycerol</scene> (C3H8O3) also known as GOL, is a ligand used for cryoprotection during the purification process of the protein. It is supposedly helping the main ligand to reach its binding site. | ||
To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene> | To do so, GOL links to<scene name='60/604479/Gol_binding_residues/1'> Asn 41 and Tyr 102.</scene> ('''Fig.2''') | ||
[[Image:Cl_Ligplot.png|right|thumb|'''Fig.3''' Cl Ligplot]] | [[Image:Cl_Ligplot.png|right|thumb|'''Fig.3''' Cl Ligplot]] | ||
*<scene name='60/604479/Cl/1'>Chloride ion</scene> facilitates the binding of other ligands to the protein. Its abundance around ASP1 varies according to changing pH conditions. At pH 5.5, <scene name='60/604479/Cl_binding_residue/1'>Val 65</scene> is the only amino acid able to fix a chloride ion. | *<scene name='60/604479/Cl/1'>Chloride ion</scene> facilitates the binding of other ligands to the protein. Its abundance around ASP1 varies according to changing pH conditions. At pH 5.5, <scene name='60/604479/Cl_binding_residue/1'>Val 65</scene> is here the only amino acid able to fix a chloride ion. ('''Fig.3''') | ||
However, in natural conditions, binding ligands are the pheromones secreted by the queen such as 9-ODA. | However, in natural conditions, binding ligands are the pheromones secreted by the queen such as 9-ODA. | ||
