Sandbox Reserved 958: Difference between revisions
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This protein is divided in three main domains: the N-terminal, the central and the C-terminal domain. | This protein is divided in three main domains: the N-terminal, the central and the C-terminal domain. | ||
The '''N-terminal domain''' (1-49) presents a HHCC motif which is a pseudo zinc-finger complexing with zinc ions. The zinc ejection impedes the 3'-processing process and pertubs the integrase multimerisation<ref name="Carayon">PMID:20164093</ref>. Therefore the presence of this ion is necessary the virus life cycle. | The '''N-terminal domain''' (1-49) presents a HHCC motif which is a pseudo zinc-finger complexing with zinc ions. The zinc ejection impedes the 3'-processing process and pertubs the integrase multimerisation<ref name="Carayon">PMID:20164093</ref>. Therefore the presence of this ion is necessary for the virus life cycle. | ||
The '''central domain''' (50-213) which corresponds to the catalytic domain contains the <scene name='60/604477/Catalytic_triad/1'>catalytic triad</scene> by association of two aspartates and one glutamate residues that coordinate bivalent ions, Cd++ in this structure but Mg++ or Mn++ ''in vivo'' <ref name="Liao">PMID:21426159</ref>. There is also an analogy of structure with the transposase core domain and <scene name='60/604477/Residues_186-194/1'>residues 186-194</scene> help to interact with DNA by contact the major groove of viral and cellular DNA<ref name="Chen">PMID:10890912</ref>. This domain contains <scene name='60/604477/Structure/2'>residues</scene> between the 170-180 position involved in the packaging of the Uracil DNA glycosylase ('''UNG2''')<ref name="Zheng">PMID:23863879</ref> essential for the viral replication. | The '''central domain''' (50-213) which corresponds to the catalytic domain contains the <scene name='60/604477/Catalytic_triad/1'>catalytic triad</scene> by association of two aspartates and one glutamate residues that coordinate bivalent ions, Cd++ in this structure but Mg++ or Mn++ ''in vivo'' <ref name="Liao">PMID:21426159</ref>. There is also an analogy of structure with the transposase core domain and <scene name='60/604477/Residues_186-194/1'>residues 186-194</scene> help to interact with DNA by contact the major groove of viral and cellular DNA<ref name="Chen">PMID:10890912</ref>. This domain contains <scene name='60/604477/Structure/2'>residues</scene> between the 170-180 position involved in the packaging of the Uracil DNA glycosylase ('''UNG2''')<ref name="Zheng">PMID:23863879</ref> essential for the viral replication. | ||
