Sandbox Reserved 951: Difference between revisions
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== Function highlighted with structure == | == Function highlighted with structure == | ||
The most known reaction of luciferase is the light emission. | === The different reactions=== | ||
====Light emission==== | |||
The most known reaction of luciferase is the light emission. In this reaction, luciferase firstly synthetized luciferin-AMP from luciferin and ATP, using a Mg2+ ion to offset the negative charges of the phosphate groups. Then, luciferase turns the luciferin-AMP into oxyluciferin in an excited state thanks to a dioxygen. This step releases AMP and CO2. The excited oxyluciferin relaxes and looses a photon so light is emitted. | |||
The wavelength of the light can vary with the pH : at the physiological pH, the emitted light is green and at a lower pH, the color is red. | |||
====Fatty-acyl-CoA synthesis==== | |||
This reaction uses the luciferin-AMP which is created at the first step of light emission. But then, CoA-SH attacks the oxygen of the carboxylic group to form fatty-acyl-CoA and releases AMP. | |||
== Evolution == | == Evolution == | ||
